更新時間:2016-06-15
小鼠胚胎成纖維細胞;3T3-Swiss albino,開始培養(yǎng)保持細胞濃度在2×10 exp5個細胞/ ml和1×10 exp5和1×10 exp6個細胞/ ml之間。只用于實驗室研究。不能被用于在臨床檢測和有關于人身安全的實驗。
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小鼠胚胎成纖維細胞;3T3-Swiss albino
形態(tài)特性 成纖維細胞樣
生長特性 貼壁生長
特征特性 小鼠胚胎成纖維細胞;3T3-Swiss albino3T3細胞株是1962年Todaro G和Green H從分離的瑞士小鼠胚胎中建立的;該細胞的生長受接觸性抑制,匯合狀態(tài)的單層細胞密度為40000個細胞/平方厘米;檢測結果顯示該細胞鼠痘病毒陰性;在塑料瓶中生長較好,在某些玻璃表面上可能狀態(tài)不佳;細胞生長飽和時其密度可以達到約50000 cells/cm2。
培養(yǎng)條件 DMEM-H: Dulbecco's Modified Eagle's Medium (DME H-21 4.5g/Liter Glucose) 10%FBS
傳代方法 1:3傳代;3~4天1次。
傳代情況 C5
凍存條件 基礎培養(yǎng)基+5%DMSO+20%FBS
公司細胞廣泛用于國內(nèi)院校的細胞生物學研究,他們的很多論文都或多或少使用我們公司的細胞,作為實驗項目研究。
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